Class Action
نویسنده
چکیده
THE PRESENCE of tissue-specific antigen in various mucins has been demonstrated in a number of studies (Bara et al., 1977; Hillermanns, 1962; Nairn et al., 1962). Antibodies to mucins from alimentary tract can be readily produced and shown by immunofluorescence to localize to endosecretory parts of the epithelial cells. More recently, Gold & Miller (1978) showed that, while common gastrointestinal specific antigens are present throughout the GI tract, mucoproteins from regions neighbouring the colon have a greater structural similarity than those from GI regions distant from the colon. The antigenic characterization of mucus from the endocervix, and in particular the antigenic (lifferentiation between endocervical and endometrial mucus, has not been adequately investigated. In this commuLnication we describe the production of a specific antiserum to endocervical mucus, which can differentiate normal as well as neoplastic endocervical from endometrial tissue. Endocervical tissue was obtained from operative specimens, homogenized in phosphate-buffered saline, and I ml of the supernatant containing 1 mg of protein/ml was emulsified in an equal volume of complete Freund's adjuvant and injected i.m. into a rabbit. A second injection was given 2 weeks later and the animal bled after a further 10 days. The serum was absorbed 3 times with normal human skin and once with normal endometrial tissue, to remove non-specific staining with cervical squamAccepte(d 13 October 198(0
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ورودعنوان ژورنال:
- Environmental Health Perspectives
دوره 102 شماره
صفحات -
تاریخ انتشار 1994